Keeneland is a custom built system for measuring sarcomere length profiles and calcium transients in intact cells (cardiomyoctes).
The system includes:
1) A dedicated Nikon inverted microscope
2) A 4-axis Syskyou stage controller
3) A custom heated stage
4) A PTI deltaRamX System
5) Aurora HVSL sarcomere length monitoring
6) GiGE video monitoring
7) custom external pacing, perfusion and temperature control.
The Keeneland system is unique compared to turnkey intact cell systems in its high-throughput ability to repeatedly measure intact cells. We've recently published two papers utilizing the unique rapid acquisition allowed by this system:
Altered ventricular torsion and transmural patterns of myocyte relaxation precede heart failure in aging F344 rats. in the American Journal of Physiology - Heart and Circulatory Physiology.
Temperature and trasmural region influence functional measurements in unloaded left ventricular cardiomycytes. in Physiological Reports.